MRI-Guided Stereotactic Biopsy of Murine GBM for Spatiotemporal Molecular Genomic Assessment

نویسندگان

  • Stefanie Galbán
  • Wajd N. Al-Holou
  • Hanxiao Wang
  • Amanda R. Welton
  • Kevin Heist
  • Xin Kathy Hu
  • Roeland GW Verhaak
  • Yuan Zhu
  • Carlos Espinoza
  • Thomas L Chenevert
  • Ben A. Hoff
  • Craig J. Galbán
  • Brian D. Ross
چکیده

Brain tumor biopsies that are routinely performed in clinical settings significantly aid in diagnosis and staging. The aim of this study is to develop and evaluate a methodological image-guided approach that would allow for routine sampling of glioma tissue from orthotopic mouse brain tumor models. A magnetic resonance imaging-guided biopsy method is presented to allow for spatially precise stereotaxic sampling of a murine glioma coupled with genome-scale technology to provide unbiased characterization of intra- and intertumoral clonal heterogeneity. Longitudinal and multiregional sampling of intracranial tumors allows for successful collection of tumor biopsy samples, thus allowing for a pathway-enrichment analysis and a transcriptional profiling of RNA sequencing data. Spatiotemporal gene expression pattern variations revealing genomic heterogeneity were found.

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عنوان ژورنال:

دوره 3  شماره 

صفحات  -

تاریخ انتشار 2017